Document Details

Document Type : Thesis 
Document Title :
Phytochemical and Biological Studies of Onopordum heteracanthum
دراسة كيميائية وبيولوجية لنبات أونوبوردم هيتيراكانثم
 
Subject : faculty of Pharmacy 
Document Language : Arabic 
Abstract : The aim of the current study was to isolate and identify the active metabolites of Onopordum heteracanthum (Family Asteraceae) and evaluate their cytotoxic activity against three cell lines: MCF-7 (breast adenocarcinoma), HepG2 (hepatocellular carcinoma) and HCT-16 (colorectal adenocarcinoma). The phytochemical study of the methanolic extract of the aerial parts of Onopordum heteracanthum led to isolation and identification of six known compounds named: β-amyrin-3-O-stearate (1), taraxasterol (2), lupeol (3), 27-nor-3β-hydroxy-25-oxocycloartane (4), stigmasterol (5) and scutellarein-6-methyl ether (6). Their structures were determined based on their spectral data: 1D (1H and 13C NMR), 2D NMR (HMBC) as well as, comparison with literature data. Their cytotoxicity was assessed towards MCF-7, HepG2 and HCT-16 cancer cell lines by sulphorhodamine B test (SRB). It is remarkable that compound 4 has high cell killing effect against MCF-7, HepG2 and HCT-116 cells with IC50 (2.1 ± 0.06 μM, 2.5 ± 0.06 μM and 2.9 ± 0.05 μM) respectively in comparison with doxorubicin IC50 (0.18 ±0.005, 0.6 ± 0.050, and 0.2 ± 0.008 μM,) respectively. 
Supervisor : Prof. Gamal Abdallah Mohamed Hussein 
Thesis Type : Master Thesis 
Publishing Year : 1443 AH
2022 AD 
Added Date : Saturday, January 28, 2023 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
نجود عبدالسلام الحربيAlharbi, Njood AbdulsalamResearcherMaster 

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