Document Details

Document Type : Thesis 
Document Title :
DETERMINATION OF HLA ALLOIMMUNIZATION IN SICKLE CELL ANEMIA
تحديد المناعة المتباينة لمستضدات الكريات البيضاء البشرية في فقر الدم المنجلي
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Background: Sickle cell anemia (SCA) is an autosomal recessive disorder that is characterized by the inheritance of the hemoglobin S gene. It is one of the most common genetic disorders in Saudi Arabia. Blood transfusion is essential in the management of SCA patients. Therefore, multiple transfusions can induce alloimmunization to foreign human leukocyte antigens (HLA) causing hemolytic transfusion reactions and burdening subsequent transfusions. In this study, HLA genotype frequencies among sickle cell disease (SCD) patients were determined and the frequency of HLA antibodies among SCD patients was estimated. Methods: A total number of 85 samples from SCD patients with multi-transfusion (n > 3) from Jeddah were recruited in this study. EDTA samples were collected for HLA genotyping (class I/II) using a molecular method (PCR-SSOP) and plasma was used for HLA antibody testing (class I/II) using a solid phase assay method (Luminex). 38 healthy population with no transfusion history were used as a control. Data were analyzed using Chi-square and Fischer exact tests using SPSS software. Results with p-value <0.05 were considered significant. Results: In SCD patients, HLA-A*02 (35.9%) type was the most common HLA-A locus, HLA- B*51 (13.5%) was the most HLA-B, HLA-C*07 (23.5%) was the most common HLA-C locus while in the control group the HLA-A*02 (39.5%), HLA-B*51 (19.7%), HLA-C*06 (28.9%) were the most common. For HLA class II, HLA-DRB1*03 (22.4%) and HLA-DQB1*02 (39.4%) were the most common between SCA patients while in the control group HLA-DRB1*04 (23.7%) and DQB1*02 (40.8%) were most common. In SCD patients, HLA antibody testing showed that 61.2% (n=52/85) of the patients were positive for HLA class I antibodies and 43.5% (n= 37/85) were positive for class II. A positive significant association was observed between the number of transfusions and HLA antibody formation at p-value <0.001. Conclusion: The incidence of HLA class I antibodies is more prevalent than HLA class II in transfused SCD patients. The number of blood transfusion was associated with an increase in HLA alloantibody formation. HLA genotyping for SCD patients can be used routinely to select HLA match unites as a strategy to reduce HLA sensitization. 
Supervisor : Dr. Talal Qadah 
Thesis Type : Master Thesis 
Publishing Year : 1442 AH
2021 AD 
Added Date : Monday, May 31, 2021 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
شروق نجيب العدوانيAl-Adwani, Shurooq NajeebResearcherMaster 

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