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Document Type |
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Thesis |
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Document Title |
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Can Vitamin E and 5-Aminosalicylic Acid ameliorate Acrylamide-induced Histological Changes in Sciatic Nerve and Frontal Cerebral Cortex of Rat? هل يمكن لفيتامين هـ و حمض الساليساليك تحسين التغيّرات النسيجية المحدثة بالأكريلاميد في العصب الوركي و قشرة الدماغ الأمامية في الجرذ؟ |
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Subject |
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Faculty of Medicine |
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Document Language |
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Arabic |
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Abstract |
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Acrylamide (AA) is a chemical substance that is metabolized in liver into glycidamide with reported toxic effects. It is formed in carbohydrate food prepared at high temperature. Neurotoxicity is the most common complication of acrylamide exposure in human and experimental animals based on oxidative stress. Neuroprotective activities of Vitamin E (Vit-E) and 5-Aminosalicylic acid (5-ASA) encouraged the design of this study to investigate their possible ameliorating effects against acrylamide-induced histological alteration in sciatic nerve and frontal cerebral cortex in rat model. Twenty five adult male rats of Wistar strain (200-250gm) were divided into 5 groups (n=5). G1: control. G2: AA, received pure AA (50 mg/kg/b.w./day) via oral gavage. G3: AA +Vit.E(200mg/kg/b.w./day). G4:AA+5-ASA (25mg/kg/b.w./day). G5: AA+ mix of Vit-E and 5-ASA(in similar doses). Experiment last for 7 consecutive days. Body weights were recorded. Gait score was assesed. Sciatic nerve and frontal cortex were dissected, fixed and processed for routine light and electron microscopic examination. Haematoxylin and Eosin, osmic acid for myelin sheath and toluidine blue for semthin section were used in addtion to immunohistochemistry for caspase-3, induced Nitric oxide synthase and Glial Fibrillary Acidic Protein. Body weight was not affected. Abnormal gait score was recorded in AA group with significant improvement in Vit-E and mixed groups. Histologically, Vit-E and 5-ASA provided potential protection against decreased sciatic nerve axon density, disrupted myelination and alteration in immunohistochemistry induced by acrylamide. Similar protection aginst neuronal apoptosis and astrocyte alteration were observed in frontal cortex and the related white matter. Vit-E and its mixture with 5-ASA provided more evident protection compared to 5-ASA. Conclusion: Vit-E and its mixture with 5-ASA showed potential protection against AA induced periphral and central neurotoxicity. It could be advised as a supplement for those exposed to AA in industrial field. However, avoiding intake of AA rich food is mandatory. Key words: Acrylamide toxicity,Vitamin E, 5-ASA, sciatic nerve, frontal cerebral cortex, histology, immunohistochemistry. |
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Supervisor |
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Prof. Soad Shaker A. Abdulhadi |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Co-Supervisor |
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Dr. Rasha Abdulrahman Al-Shali |
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Added Date |
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Tuesday, August 27, 2019 |
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Researchers
| فاطمة ريس محفوظ | mahfouz, Fatmah Rais | Researcher | Master | |
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